Must have thorough understanding of the theories, practice and techniques used in histology to include fixation, decalcification, processing, embedding, microtomy, routine and special stains, immunohistochemistry staining and frozen sections. Must be able to trouble shoot and rectify problems with minimal supervisory intervention.
Must be fully competent with all laboratory instrumentation to include pH meter, balances, microtomes, tissue processors, cryostats, coverslippers, etc.
Must be able to prepare percent, molar and normal solutions and stains.
Perform routine immunohistochemical stains both manually and on automated instrumentation as required.
Fixes tissues in appropriate fixatives; dehydrates with graded alcohols; clears with xylene; impregnates with paraffin wax using an automated tissue processor.
Embed specimens ensuring proper orientation. Produces 4 micron sections using a microtome and appropriate knives; floats sections on a water bath; collects sections on a glass slide; dries in a slide dryer for a specified time.
Stains slides, with routine and special stains according to approved procedures to enhance certain tissue elements required for diagnosis. Coverslips and labels stained slides. Examines slides with a microscope to ascertain that the results are satisfactory.
Ensures all blocks and slides are clerically correct. Assembles and collates slides. Distribute slides to residents and staff pathologists.
Determines when prescribed techniques and apparatus should be modified in order to meet required quality standards.
Prepares specimens for a rapid frozen section diagnosis using liquid nitrogen and a cryostat microtome. Stains frozen sections using various stains.
Shares responsibility for preparation of solutions and reagents in accordance with approved procedures. Maintains quality control records. Keeps accurate records of blocks and slides produced. Files tissue blocks, ensuring each is properly labeled.
Other Duties as assigned.
Must be registered HT(ASCP) or HLT(ASCP)
Minimum of a high school degree or graduate of a NCCALS approved school of Histotechnology or Associates Degree; Bachelors Degree preferred.
Three to four years clinical experience, or five to six years histology experience in a research setting
Internal Number: 3132086
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