We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.
We don’t follow footsteps. We create the path.
This individual will be responsible for processing tissues for digital histopathology and maintaining the daily operations in the histology core at Spark Therapeutics. The candidate will use image acquisition and analysis to support all of Spark’s therapeutic areas.
Functions include all phases of tissue preparation, including fresh or in-process specimens (paraffin and frozen)
Routine paraffin and cryo embedding and sectioning, H&E (Hematoxylin & Eosin) staining, special stains technique (histochemistry), immunohistochemistry, microscopic, and image analysis are required.
Responsible for examining tissues upon receipt and applying understanding of gross anatomy and study purpose to determine and dissect appropriate representative samples for histology testing and Pathology analysis.
Specimen preparation that requires a complex and thorough understanding of tissue structure, as well as a basic understanding of chemistry and physics; and how tissues react under process conditions, including decalcification, rehydration, grossing, fixation, embedding, microtomy, routine staining, special stains (histochemistry), and immunohistochemistry.
Develop and drive histology-based assays (through external and internal collaboration) including immunohistochemistry (IHC), immunofluorescence (IF) and in-situ hybridization (ISH). Capable of troubleshooting and identifying artifacts.
Perform in-house qualitative or quantitative image analysis across different tissue staining platforms; would be familiar with common digital imaging software (i.e. FIJI/ImageJ, HALO from Indica Labs, etc.)
Summarize findings (PowerPoint presentation, research reports etc.) and communicate to peers and management and wider Research and Clinical groups as projects evolve
This individual will also be responsible for maintenance, operation, qualification/validation of histology equipment, as well as training technicians and peers in histological sample handling, histochemical and immunohistochemical technique, imaging and analysis.
Hands-on Tissue processing, staining and image acquisition
Participate in investigations
Review and revise SOPs
Maintain equipment and records
Maintain laboratory safety procedures and compliance records
• Prepare laboratory reagents/materials
Education and Experience Requirements:
PhD, DVM, or Masters degree in Biology, immunology, pathology, or related fields; B.S. will be considered with equivalent experience
Generally has 3-5 years of relevant pre-clinical experience
Experience in histology-based biomarker assay development and validation
Experience performing and troubleshooting singleplex and multiplex IHC, IF, and/or ISH assays
Experience in applying DNA/RNA profiling techniques in formalin-fixed tissue is preferred.
Excellent interpersonal and communication skills as well as the ability to work effectively as part of a cross functional team
Key Skills, Abilities, and Competencies:
Strong organizational skills.
Excellent writing and verbal communication skills.
Independently motivated, detail oriented and good problem solving ability.
Ability to multitask.
Ability to analyze and troubleshoot experiments.
Complexity and Problem Solving:
Demonstrate independent problem solving skills of technical issues; develop solutions to a variety of moderate to complex problems; Discuss with supervisor and refer to policies/practices for guidance.
Must be comfortable in the fast-paced, biotech start-up environment and interact with multi-disciplinary research teams at Spark.
Exercise judgment within defined procedures and practices to determine appropriate action.
Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.
We Are Spark
At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.
Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.
Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-...time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.
Challenge the inevitability of genetic disease by discovering, developing and delivering treatments in ways unimaginable – until now.
A world where no life is limited by genetic disease.